To　determine　the　effect　of　vitamin　supplements　on　the　oral　bioaccessibility　of　Pb　in　soils,　Pb　bioaccessibility　was　measured　in　the　presence　of　9　vitamins　by　a　physiologically　based　extraction　test.　Gastric　Pb　bioaccessibility　(G-BA,　2.6–83.3%)　was　found　to　be　mostly　reduced　(1.1–3.1　fold)　in　the　presence　of　B　vitamins,　specifically　vitamins　B1,　B6,　and　B9.　In　contrast,　a　significant　increase　in　Pb　G-BA　was　observed　with　vitamin　C　and　E　involved.　In　the　small　intestinal　phases,　Pb　bioaccessibility　(I-BA)　ranged　from　0.1%　to　16.0%,　being　5–50　fold　lower　than　the　corresponding　G-BA　values.　Vitamin　C　supplementation　showed　a　7-fold　increase　in　Pb　I-BA,　with　a　similar　increase　presented　in　approximately　30%　of　samples　treated　to　vitamin　B　involvement.　Lead　liberation　in　gastrointestinal　digests　was　associated　with　the　dissolution　of　Fe　and　Mn　regulated　by　vitamins.　In　conclusion,　the　addition　of　B　vitamins　resulted　in　the　reduction　of　gastric　Pb　bioaccessibility,　but　the　bioaccessibility　value　increased　in　participation　of　vitamin　C　and　E.　Elevated　intestinal　bioaccessibility　was　found　especially　for　vitamin　C.　This　should　contribute　to　more　accurate　assessment　of　health　risks　from　contaminated　soils.　Nutritional　management　aimed　at　preventing　Pb-induced　toxicity　can　benefit　from　knowledge　of　vitamin　influence　on　soil　Pb　bioaccessibility.　©　2020　Elsevier　B.V.