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author:

Feng, J. (Feng, J..) [1] | Ma, Y.-L. (Ma, Y.-L..) [2] | Sun, P. (Sun, P..) [3] | Thakur, K. (Thakur, K..) [4] | Wang, S. (Wang, S..) [5] | Zhang, J.-G. (Zhang, J.-G..) [6] | Wei, Z.-J. (Wei, Z.-J..) [7]

Indexed by:

Scopus

Abstract:

Enzymatically derived peptides from different proteins have displayed the potential to provide health benefits. Present research aims to obtain the functional peptides and improve the economic value of camellia seed cake via enzymatic hydrolysis. Camellia seed cake protein hydrolysates (CSCPH) prepared with alcalase demonstrated higher α-glucosidase inhibitory activity. After ultrafiltration, the α-glucosidase inhibitory activity of different components was compared. The component (CSCPH-IV) with the highest activity was selected for further separation and purification. The peptides were purified using reverse-phase high-performance liquid chromatography (RP-HPLC) twice and identified as GHSLESIK, GLTSLDRYK and SPGYYDGR by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Subsequently, the preliminarily inhibitory effect of these peptides on α-glucosidase activity was demonstrated by the molecular docking model. These results showed that three novel peptides isolated from the hydrolysates of camellia seed cake have the potential to be used as anti-diabetic compounds for the development of functional foods. © 2020 Institute of Food Science and Technology

Keyword:

Camellia seed cake; protein hydrolysate; purified peptides; α-Glucosidase inhibitory activity

Community:

  • [ 1 ] [Feng, J.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 2 ] [Ma, Y.-L.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 3 ] [Sun, P.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 4 ] [Thakur, K.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 5 ] [Wang, S.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
  • [ 6 ] [Zhang, J.-G.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 7 ] [Wei, Z.-J.]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China
  • [ 8 ] [Wei, Z.-J.]Biological Science and Engineering College, North Minzu University, Yinchuan, 750021, China

Reprint 's Address:

  • [Ma, Y.-L.]School of Food Science and Biological Engineering, Hefei University of TechnologyChina

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Source :

International Journal of Food Science and Technology

ISSN: 0950-5423

Year: 2020

2 . 6 0 0

JCR@2023

ESI HC Threshold:116

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 26

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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