Surface　landscapes　have　vague　impact　on　the　mechanical　properties　of　graphene.　In　this　paper,　single-layered　graphene　sheets　(SLGS)　with　regular　wrinkles　were　first　constructed　by　applying　shear　deformation　using　molecular　dynamics　(MD)　simulations　and　then　indented　to　extract　their　mechanical　properties.　The　influence　of　the　boundary　condition　of　SLGS　were　considered.　The　wrinkle　features　and　wrinkle　formation　processes　of　SLGS　were　found　to　be　significantly　related　to　the　boundary　conditions　as　well　as　the　applied　shear　displacement　and　velocity.　The　wrinkling　amplitude　and　degree　of　wrinkling　increased　with　the　increase　in　the　applied　shear　displacements,　and　the　trends　of　wrinkling　wavelengths　changed　with　the　different　boundary　conditions.　With　the　fixed　boundary　condition,　the　degree　of　graphene　wrinkling　was　only　affected　when　the　velocity　was　greater　than　a　certain　value.　The　effect　of　wrinkles　on　the　mechanical　characterization　of　SLGS　by　atomic　force　microscopy　(AFM)　nanoindentation　was　finally　investigated.　The　regular　surface　wrinkling　of　SLGS　was　found　to　weaken　the　Young＇s　modulus　of　graphene.　The　Young＇s　modulus　of　graphene　deteriorates　with　the　increase　in　the　degree　of　regular　wrinkling.　©　2020　by　the　authors.