A　new　folic　acid　(FA)-conjugated　poly　(lactic-co-glycolicacid)　(PLGA)-polyethylene　glycol　(PEG)　nano-noisome　was　prepared.　The　noisome　was　employed　as　a　drug　delivery　system　to　load　curcumin　(Cur)　as　a　model　drug　and　fluorescent　probe　for　cervical　cancer　therapy　and　cell　imaging.　The　Fe3O4@PLGA-PEG@FA　noisomes　were　prepared　through　facile　emulsion　solvent　evaporation　and　conjugation　chemistry　method,　possessing　the　properties　of　high　rapid　magnetic　separation　and　targeting　character.　X-ray　photoelectron　spectroscopy　(XRD),　Fourier　transform　infrared　spectroscopy　(FTIR),　dynamic　light　scattering　(DLS),　thermogravimetric　analysis　(TGA),　and　vibrating　sample　magnetometer　(VSM)　were　adopted　to　characterize　the　chemical　structure　and　properties　of　these　niosomes.　MTT　assay　revealed　that　the　blank　noisomes　exhibited　excellent　biocompatibility.　The　in　vitro　drug　loading　and　release　behavior　studier　showed　the　as　prepared　nano-noisome　presented　ultrahigh　performance　as　drug　carrier.　The　confocal　laser　scanning　microscopy　(CLSM)　and　flow　cytometry　(FCM)　experiments　demonstrated　that　Cur-loaded　Fe3O4@PLGA-PEG@FA　niosomes　achieved　significantly　high　targeting　efficiency　for　cervical　cancer.　Additionally,　the　FA-targeted　niosomes　exhibited　higher　antitumor　efficiency　than　free　Cur.　Cell　morphology,　the　mitochondrial　membrane　potential　and　cell　cycle　changes　indicated　that　Cur-loaded　niosomes　induced　HeLa229　cells　to　apoptosis　by　destroying　mitochondrion　of　cervical　tumor　cells,　simultaneously　changing　nuclear　morphology　and　blocking　tumor　cell　proliferation.　These　results　demonstrate　that　Fe3O4@PLGA-PEG@FA　noisomes　have　promising　applications　as　targeted　drug　delivery　system　for　sustained　drug　release　in　cancer　treatment.　©　2018　Elsevier　B.V.