To　satisfy　the　demands　for　production　peak,　reduce　personnel　costs　for　labor,　limit　the　increase　of　employees　in　enterprises,　and　focus　on　corporate　specialty　to　develop　the　competitive　advantage,　enterprises　would　generally　apply　human　resource　flexibility　strategy　to　achieve　the　objectives.　The　practice　of　human　resource　flexibility　strategy　would　change　work-related　characteristics;　besides,　the　effect　of　the　system　on　employees　would　decide　the　effort,　absenteeism,　or　turnover.　Aiming　at　supervisors　and　employees　in　ecotourism,　as　the　research　objects,　total　500　copies　of　questionnaire　are　distributed,　and　351　valid　copies　are　retrieved,　with　the　retrieval　rate　70%.　The　research　results　reveal　positive　and　significant　effects　of　1.human　resource　flexibility　strategy　on　organizational　citizenship　behavior,　2.organizational　citizenship　behavior　on　organizational　performance,　and　3.human　resource　flexibility　strategy　on　organizational　performance.　According　to　the　results,　suggestions　are　eventually　proposed,　expecting　to　provide　essential　assistance　for　the　human　resource　flexibility　strategy　in　ecotourism　and　assist　in　the　sustainable　development.　©　Foundation　Environmental　Protection　&　Research-FEPR.