As　signal　molecules,　auxins　play　an　important　role　in　mediating　plant　growth.　Due　to　serious　interfering　substances　in　plants,　it　is　difficult　to　accurately　detect　auxins　with　traditional　solid-phase　extraction　methods.　To　improve　the　selectivity　of　sample　pretreatment,　a　novel　molecularly　imprinted　polymer　-coated　solid-phase　microextraction　fiber,　which　could　be　coupled　directly　to　high-performance　liquid　chromatography,　was　prepared　with　indole　acetic　acid　as　template　molecule　for　the　selective　extraction　of　auxins.　The　factors　influencing　the　polymer　formation,　such　as　polymerization　solvent,　cross-linker,　and　polymerization　time,　were　investigated　in　detail　to　enhance　the　performance　of　indole　acetic　acid-molecularly　imprinted　polymer　coating.　The　morphological　and　chemical　stability　of　this　molecularly　imprinted　polymer-coated　fiber　was　characterized　by　scanning　electron　microscopy,　infrared　spectrometry,　and　thermal　analysis.　The　extraction　capacity　of　the　molecularly　imprinted　polymer-coated　solid-phase　microextraction　fiber　was　evaluated　for　the　selective　extraction　of　indole　acetic　acid　and　indole-3-pyruvic　acid　followed　by　high-performance　liquid　chromatography　analysis.　The　linear　range　for　indole　acetic　acid　and　indole-3-pyruvic　acid　was　1–100 µg/L　and　their　detection　limit　was　0.5 µg/L.　The　method　was　applied　to　the　simultaneous　determination　of　two　auxins　in　two　kinds　of　tobacco　(Nicotiana　tabacum　L　and　Nicotiana　rustica　L)　samples,　with　recoveries　range　from　82.1　to　120.6%.　©　2019　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim