The　high-throughput　and　rapid　screening　of　cancer　cells　in　blood　is　one　of　the　most　effective　ways　to　realize　clinical　early　diagnosis　of　cancer.　We　herein　developed　an　inductively　coupled　plasma　mass-spectrometry　(ICP-MS)-based　method　for　simultaneously　counting　two　cancer　cells,　human　hepatocellular　carcinoma　cell　(SMMC-7721)　and　human　lung　carcinoma　cell　(A549),　with　high　sensitivity　and　specificity.　The　method　employed　a　magnetic-beads　(MBs)-based　dual　aptamers-dual　metal　nanoparticles　labeling　technique　for　simultaneously　recognizing　and　labeling　different　metal　nanoparticles　(AuNPs　and　AgNPs)　on　SMMC-7721　and　A549　cancer　cells,　respectively,　to　generate　ICP-MS　signals,　and　a　DNA　hybridization　chain　reaction　strategy　for　realizing　＂one　SMMC-7721　cell-to-massive　AuNPs＂　and　＂one　A549　cell-to-massive　AgNPs＂　amplification　effect　to　improve　sensitivity.　The　employment　of　ICP-MS　detection　and　MBs　not　only　simplified　the　experimental　operation　but　also　greatly　enhanced　the　resistance　of　the　method　to　the　complicated　matrix.　The　method　can　be　used　to　simultaneously　detect　as　few　as　50　SMMC-7721　and　A549　cancer　cells　in　serum　within　1　h　with　a　recovery　of　93-108%　and　a　relative　standard　deviation　(RSD,　n　=　5)　＜　5%.　The　method　has　notable　advantages　such　as　high　sensitivity,　excellent　stability,　high　throughput,　shorter　analysis　time,　and　strong　resistibility　to　the　complex　matrix.　Especially,　the　method　can　also　be　used　to　detect　various　cancer　cells　via　altering　aptamers　and　designing　appropriate　link/signal　probes　according　to　the　target　cells.　The　success　of　this　study　offers　a　potential　approach　for　the　high-throughput　and　rapid　screening　of　cancer　cells　in　clinical　early　diagnosis　of　cancer.　©　2018　American　Chemical　Society.