Bovine　serum　albumin　(BSA)　imprinted　polyampholyte　hydrogels　(PAHs)　were　prepared　by　free　radical　polymerization　using　acrylamide　(Am)　as　structural　monomer,　N-isopropylacrylamide　(NIPAm),　[2-(methacryloyloxy)ethyl]trimethylammonium　chloride　(DMC)　and　2-acrylamido-2-methyl-1-propanesulfonic　acid　(AMPS)　as　functional　monomers　and　N,N′-methylenebisacrylamide　(MBA)　as　crosslinker　in　aqueous　solution.　The　morphology　of　imprinted　hydrogels　and　non-imprinted　hydrogels　were　characterized　by　scanning　electron　microscope　(SEM).　The　adsorption　and　recognition　properties　were　evaluated　as　functions　of　Am　monomer　concentration,　NIPAm/Am　molar　ratio,　crosslinking　structure　and　charge　density　ratio　etc.　The　adsorption　capacity　and　association　constant　of　specific　interaction　between　hydrogel　and　template　protein　were　analyzed　by　Langmuir　isotherm　model　and　Freundlich　model.　The　fitting　experimental　data　suggested　that　this　adsorption　was　better　described　as　a　monolayer　adsorption.　The　specific　adsorption　on　hydrogel　with　different　crosslinking　structure　was　investigated　by　selective　binding　BSA　from　single　solution　and　binary　mixture　solution.　The　charge　density　ratio　in　molecular　imprinting　hydrogel　had　obvious　influence　for　protein　adsorption　and　recognition.　The　resultant　of　regeneration　tests　showed　that　elution　had　large　impact　on　deterioration　of　the　imprinting　structure.　©　2018　Elsevier　B.V.