Magnetic-responsive　materials　are　promising　for　applications　in　various　biomedical　fields.　Especially,　superparamagnetic　nanoparticles　are　widely　used　in　magnetic　system　for　bone　tissue　engineering　owing　to　superior　biocompatibility　and　long　term　stability.　Based　on　the　idea　of　in　situ　bionics,　we　successfully　incorporate　the　nano-hydroxyapatite　(nHAP)　and　Fe　3　O　4　nanoparticles　which　were　prepared　by　in　situ　crystallization　and　freeze-drying　technique　into　the　chitosan/collagen　(CS/Col)　organic　matrix　to　achieve　the　uniform　dispersion　of　inorganic　substrate　with　nanometer-scale.　The　in　vitro　results　of　the　physicochemical　and　biocompatibility　tests　showed　that　CS/Col/Fe　3　O　4　/nHAP　magnetic　scaffold　possessed　superior　structural　and　mechanical　performance　for　cell　adhesion　and　proliferation,　as　well　as　the　osteogenic　differentiation.　Mineralization　experiments　showed　better　bioactive　and　good　ability　of　in　situ　biomimetic　mineralization.　Moreover,　from　the　in　vivo　model　of　SD　rats’　skull　defects　proved　that　the　CS/Col/Fe　3　O　4　/nHAP　hybrid　scaffold　had　a　better　tissue　compatibility　and　higher　bone　regeneration　ability　when　implanted　into　the　skull　defects　comparing　to　control　group.　Herein,　the　magnetic　hybrid　micro/nanostructured　scaffold　showed　a　potential　application　for　bone　defect　repair.　©　2018　Elsevier　B.V.