Herein,　an　autonomous　table-tennis-motion-type　DNA　machine　(ATT　DNA　machine)　was　proposed　to　integrate　target　recycling　amplification　(TRA)　and　triple　strand-displacement　amplification　(T-SDA)　into　one　signaling　system　for　the　efficient　amplification　detection　of　p53　gene.　Firstly,　X-shaped　DNA　duplex　probe　(XSP)　was　formed　via　the　simple　annealing　of　Probe　A　and　Probe　B.　Upon　addition　of　p53,　the　hybridization　of　p53　with　Probe　A　results　in　the　peeling　of　Probe　B　from　XSP.　Then,　the　primer　binds　to　the　Probe　B　to　trigger　a　circular　strand-displacement　amplification,　during　which　new　primers　are　generated.　Moreover,　the　newly-produced　primers　can　in　turn　activate　the　target　recycling　amplification　and　triple　strand-displacement　amplification　via　bouncing　back　and　forth　between　different　Probe　As　and　Probe　Bs　in　a　manner　analogous　to　the　table　tennis　motion.　This　newly-developed　DNA　machine　can　be　used　to　detect　p53　down　to　0.5　pM　with　an　excellent　specificity,　offering　a　100-fold　enhanced　sensitivity　compared　to　a　non-amplification　one-to-one　hybridization　based　system.　Moreover,　this　DNA　machine　is　able　to　be　applied　for　target　analysis　in　complex　environments.　Additionally,　the　potential　for　practical　applications　is　also　verified,　demonstrating　a　promising　alternative　tool　for　early　diagnosis　of　cancers.　©　2018　Elsevier　B.V.