The　study　aimed　to　extend　the　applications　of　surface-enhanced　Raman　scattering　(SERS)　–　recently　one　of　the　most　promising　and　widely　employed　technique　in　food　safety　and　quality　monitoring　–　towards　determination　of　two　analytes　in　a　single　step　for　the　first　time.　Thus,　an　ultra-sensitive　and　selective　SERS　based　detection　platform　for　the　simultaneous　determination　of　pesticides　namely　acetamiprid　(AC)　and　2,4-dichlorophenoxyacetic　acid　(2,4-D)　in　matcha　tea　has　been　developed.　Owing　to　the　potential　interference　of　different　nutrients　and　pigments　in　complicated　matrices,　the　matcha　tea　samples　with　and　without　spiking　AC　and　2,4-D,　were　percolated　through　a　solid　phase　extraction　cartridge.　The　eluate　was　introduced　to　the　SERS　Au@Ag　nanostructure　substrate　for　monitoring　SERS　signal　with　subsequent　chemometrics　modeling.　Under　the　optimized　experimental　conditions,　a　linear　calibration　exhibited　at　GA-PLS　model　with　RPD　values　of　6.53　and　6.23,　and　Rp　values　0.9943　and　0.9923,　respectively　for　AC　and　2,4-D.　The　method　was　validated　in　both　mixed　and　single　spiking　conditions　successfully　in　terms　of　student＇s　t-　and　F–　test　at　95%　confidence　with　satisfactory　recovery　percentages.　An　RSD　value　＜　4.85%　indicates　good　reproducibility　of　the　proposed　method.　©　2019　Elsevier　Ltd