In　this　paper,　we　propose　a　promising　super-resolution　imaging　scheme　in　fluorescence　lifetime　domain　(lifetime　super-resolution　optical　fluctuation　imaging,　ltSOFI).　ltSOFI　has　the　potential　to　obtain　super-resolution　images　by　taking　advantage　of　fluorescence　lifetime　blinking　under　wide-field　lifetime　detection.　The　proof-of-concept　for　ltSOFI　was　demonstrated　through　numerical　simulation　of　high-order　cumulant　analysis　on　fluorescence　lifetime　blinking　emitters.　As　a　tentative　experimental　demonstration,　we　obtained　super-resolution　lifetime　imaging　from　time-lapse　FLIM　recording　of　HeLa　cells　expressing　a　cAMP　sensor　using　ltSOFI　method.　ltSOFI　is　expected　to　initiate　a　new　dimension　in　the　lifetime　domain　for　blinking-based　super-resolution　microscopy.　Lay　Description:　We　report　on　a　promising　super-resolution　imaging　scheme　in　fluorescence　lifetime　domain　(lifetime　super-resolution　optical　fluctuation　imaging,　ltSOFI).　ltSOFI　has　the　potential　to　obtain　super-resolution　images　by　taking　advantage　of　fluorescence　lifetime　blinking　under　wide-field　lifetime　detection.　Past　advances　in　super-resolution　fluorescence　microscopy　primarily　rely　on　the　spatiotemporal　modulation　of　the　fluorescence　intensity.　Although　the　applications　of　the　Q-dot　blinking　have　been　discussed　in　the　literature,　most　of　the　discussions　have　focused　on　the　blinking　of　fluorescence　intensity.　Few　studies　have　shown　the　possibility　of　super-resolution　imaging　through　fluorescence　lifetime　fluctuations.　In　this　paper,　we　proposed　the　ltSOFI　scheme　that　explored　the　possibility　of　super-resolution　reconstruction　from　the　blinking　of　fluorescence　lifetime.　The　proof-of-concept　for　ltSOFI　was　demonstrated　through　numerical　simulation　of　high-order　cumulant　analysis　on　fluorescence　lifetime　blinking　emitters.　As　a　tentative　experimental　demonstration,　we　obtained　super-resolution　lifetime　imaging　from　time-lapse　FLIM　recording　of　HeLa　cells　expressing　a　cAMP　sensor　using　ltSOFI　method.　The　ltSOFI　method　is　expected　to　initiate　a　new　dimension　in　the　lifetime　domain　for　blinking-based　super-resolution　microscopy.　Moreover,　the　existing　fluorescence　lifetime　imaging　microscopy　and　super-resolution　nanoscopy　can　benefit　from　the　implementation　of　ltSOFI　to　significantly　improve　the　imaging　spatial　resolution　of　fluorescence　lifetime　images.　In　addition,　the　proof-of-concept　demonstration　achieved　by　the　numerical　simulation　and　tentative　experiment　will　provide　a　new　perspective　for　obtaining　fluorescence　lifetime　images　with　much　finer　details.　©　2019　The　Authors　Journal　of　Microscopy　©　2019　Royal　Microscopical　Society