Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR - Details

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Abstract：

This　study　developed　an　ultrasensitive　method　involving　(1)　gold　nanoparticles　encoded　with　a　double-stranded　DNA　as　the　first　signal　amplification　and　goat　anti-staphylococcal　enterotoxin　B　(SEB)　polyclonal　antibody　and　(2)　magnetic　microparticles　coated　with　anti-SEB　monoclonal　antibody　to　detect　SEB.　Both　functionalized　nanoparticles　can　capture　SEB　in　a　sandwich　system.　The　released　DNA　barcodes　were　then　characterized　through　SYBR　Green　real-time　polymerase　chain　reaction　and　resulted　in　the　second　signal　amplification.　Under　optimized　experimental　conditions,　an　ultrasensitive　bio-barcode　for　SEB　detection　was　established,　and　its　detection　limit　could　reach　0.269　pg　mL　−1　,　which　was　1000-fold　lower　than　that　of　conventional　enzyme-linked/immunosorbent　assay.　The　proposed　method　shows　great　potential　in　food　security.　©　2019　Elsevier　Ltd

Keyword：

Bio-barcode assay; Real-time PCR; Staphylococcal enterotoxin B

Community：

[ 1 ] [Xu, Y.]College of Food Science and Engineering, Jilin University, Changchun, 130022, China
[ 2 ] [Huo, B.]College of Food Science and Engineering, Jilin University, Changchun, 130022, China
[ 3 ] [Li, C.]Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin, 300050, China
[ 4 ] [Peng, Y.]Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin, 300050, China
[ 5 ] [Tian, S.]School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin, 300050, China
[ 6 ] [Fan, L.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Bai, J.]Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin, 300050, China
[ 8 ] [Ning, B.]Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin, 300050, China
[ 9 ] [Gao, Z.]Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin, 300050, China

Reprint 's Address：

[Xu, Y.]College of Food Science and Engineering, Jilin UniversityChina

Email：

xu_yanyang@yahoo.com

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Source ：

Food Chemistry

ISSN： 0308-8146

Year： 2019

Volume： 283

Page： 338-344

6 . 3 0 6

JCR@2019

8 . 5 0 0

JCR@2023

ESI HC Threshold：133

JCR Journal Grade：1

CAS Journal Grade：1

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WoS CC Cited Count：

SCOPUS Cited Count： 31

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 0

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