Antigens　and　antibodies　can　bind　specifically,　so　antibiotic　antibody　shows　potential　biological　and　environmental　applications　on　the　removal　of　antibiotic.　In　the　present　study,　novel　antibody　complex　was　synthesized　from　polyamide-amine　dendrimer　immobilized　tetracycline　(TC)　antibody　with　the　encapsulation　of　magnetic　Fe3O4　nanoparticles.　As-prepared　magnetic　Fe3O4-PAMAM-antibody　complexes　were　characterized　by　different　techniques　such　as　Fourier　transform　infrared　(FT-IR),　X-ray　diffraction　(XRD),　Nuclear　magnetic　resonance　(NMR)　and　ultraviolet　(UV)　analysis　spectra.　The　prepared　antibody　complexes　exhibited　high　adsorption　properties　for　TC　from　aqueous　solutions.　These　results　suggest　that　the　antibody　complex　expects　to　be　a　potential　candidate　for　the　wastewater　treatment.　©　2019　Fujian　Institute　of　Research　of　the　Structure　of　Matter.　All　rights　reserved.