Nonspecific　adsorption　is　a　challenge　of　specific　recognition　on　aptamer-based　affinity　monoliths.　Here,　a　novel　highly　hydrophilic　polyhedral　oligomeric　silsesquioxane　(POSS)-containing　aptamer-based　hybrid-silica　affinity　monolith　with　a　good　recognition　nature　was　prepared　and　used　for　specific　discrimination　of　ochratoxin　A　(OTA).　A　homogeneous　polymerization　mixture　consisted　of　POSS　chemicals,　hydrophilic　monomers　and　aptamer　solution　was　directly　polymerized　via　the　“one-pot”　method.　Preparation　and　characterization　of　the　resultant　affinity　monolith　were　studied　in　detail.　A　highly　hydrophilic　nature　was　obtained　and　the　typical　hydrophilic　interaction　liquid　chromatography　(HILIC)　was　observed　when　acetonitrile　(ACN)　content　in　mobile　phase　was　25%,　which　reached　the　highest　hydrophilicity　of　POSS-based　hybrid　monoliths.　By　using　OTA　as　model　analyte,　the　nonspecific　adsorption　was　effectively　suppressed.　The　recovery　of　the　analogue　ochratoxin　B　(OTB)　was　only　about　0.1%　even　if　the　content　of　OTB　was　50　times　more　than　OTA,　which　was　much　better　than　other　POSS-containing　monoliths　and　polar　siloxane-based　hybrid　monoliths.　Applied　to　beer　samples,　the　adsorption　of　background　materials　was　drastically　resisted,　and　efficient　recognition　of　OTA　was　obtained　with　the　recoveries　of　94.9–99.8%.　Much　less　disturbance　was　observed　than　that　occurred　in　hydrophobic　POSS-based　affinity　monolith.　It　lights　an　attractive　implement　with　high　hydrophilicity　and　specificity　for　online　selective　recognition　of　OTA.　©　2019