This　study　aimed　to　investigate　the　cryoprotective　activities　of　antifreeze　glycopeptide　analogues　(GAPP)　obtained　by　nonenzymatic　glycation　and　explore　the　mechanism　of　GAPP　on　Streptococcus　thermophilus　during　cold　stress.　Results　showed　that　the　survivability　of　S.　thermophilus　increased　from　32.03%　to　92.56%　treated　with　5　mg/mL　GAPP.　The　glass　transition　temperature　of　GAPP　increased　from　−27.6　to　−21.4　°C　after　nonenzymatic　glycation.　The　thermal　hysteresis　activity　of　GAPP　was　significantly　higher　and　the　amount　of　ice　nuclei　in　GAPP　groups　was　lower　than　that　of　BSA　and　antifreeze　peptides.　GAPP　could　significantly　inhibit　the　leakage　of　intracellular　proteins　and　nucleic　acids,　and　prevent　the　decrease　of　β-galactosidase　and　lactic　dehydrogenase　activities.　Cells　disruption　and　contents　leakage　could　be　observed　in　control　groups,　while　cells　with　GAPP　maintained　satiation　and　integrity.　The　results　suggested　that　GAPP　could　be　potentially　developed　as　a　new　and　effective　probiotics　cryoprotectant　in　frozen　food　system.　©　2019　Elsevier　Ltd