Microbial　aerobic　methane　oxidation　(MAMO)　has　been　considered　as　an　environmental-friendly　method　for　mitigating　methane　emission　from　municipal　landfill　sites.　Soil　column　has　in　a　landfill　cover　under　one-dimensional　(1-D)　condition.　However,　most　of　the　published　soil　column　tests　failed　to　simulate　1-D　heat　transfer　due　to　the　use　of　thermal　conductive　boundary　at　the　sidewall.　In　the　present　study,　a　heavily　instrumented　soil　column　was　developed　to　quantify　the　effects　of　thermal　boundary　condition　on　the　methane　oxidation　efficiency　under　different　ambient　temperatures　in　landfill　cover　soil.　The　sidewall　of　the　soil　column　was　thermally　insulated　to　ensure　1-D　heat　transport　as　would　have　been　typically　expected　in　the　field　condition.　Two　soil　column　tests　with　and　without　thermal　insulation　were　conducted　at　a　range　of　controlled　ambient　temperatures　from　15　to　30　°C,　for　studying　how　soil　moisture,　matric　suction,　gas　pressure,　soil　temperature　and　gas　concentration　evolve　with　MAMO.　The　test　results　reveal　that　ignoring　thermal　insulation　in　a　soil　column　test　would　result　in　a　greater　loss　of　soil　heat　generation　by　MAMO　and　hence　oxidation　efficiency　by　up　to　100%　for　the　range　of　temperature　considered.　When　the　ambient　temperature　increased　to　30　°C　(but　less　than　the　optimum　temperature　for　MAMO),　the　MAMO　efficiency　increased　abruptly　at　first　but　then　decreased　substantially　with　time,　and　this　is　likely　due　to　the　accumulation　of　biomass　generated　by　MAMO.　©　2019　Elsevier　B.V.