Logic-Gate-Actuated DNA-Controlled Receptor Assembly for the Programmable Modulation of Cellular Signal Transduction - Details

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Abstract：

Programming　cells　to　sense　multiple　inputs　and　activate　cellular　signal　transduction　cascades　is　of　great　interest.　Although　this　goal　has　been　achieved　through　the　engineering　of　genetic　circuits　using　synthetic　biology　tools,　a　nongenetic　and　generic　approach　remains　highly　demanded.　Herein,　we　present　an　aptamer-controlled　logic　receptor　assembly　for　modulating　cellular　signal　transduction.　Aptamers　were　engineered　as　“robotic　arms”　to　capture　target　receptors　(c-Met　and　CD71)　and　a　DNA　logic　assembly　functioned　as　a　computer　processor　to　handle　multiple　inputs.　As　a　result,　the　DNA　assembly　brings　c-Met　and　CD71　into　close　proximity,　thus　interfering　with　the　ligand–receptor　interactions　of　c-Met　and　inhibiting　its　functions.　Using　this　principle,　a　set　of　logic　gates　was　created　that　respond　to　DNA　strands　or　light　irradiation,　modulating　the　c-Met/HGF　signal　pathways.　This　simple　modular　design　provides　a　robust　chemical　tool　for　modulating　cellular　signal　transduction.　©　2019　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim

Keyword：

aptamers; DNA nanotechnology; logic gates; receptor assembly; signal transduction

Community：

[ 1 ] [Chen, S.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 2 ] [Chen, S.]Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China
[ 3 ] [Xu, Z.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 4 ] [Yang, W.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 5 ] [Lin, X.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 6 ] [Li, J.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 7 ] [Li, J.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China
[ 8 ] [Li, J.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 9 ] [Li, J.]Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China
[ 10 ] [Yang, H.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China

Reprint 's Address：

[Li, J.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou UniversityChina

Email：

lijuan@fzu.edu.cn

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Source ：

Angewandte Chemie - International Edition

ISSN： 1433-7851

Year： 2019

Issue： 50

Volume： 58

Page： 18186-18190

1 2 . 9 5 9

JCR@2019

1 6 . 1 0 0

JCR@2023

ESI HC Threshold：184

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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