Long　non-coding　RNAs　(lncRNAs)　are　non-protein　coding　transcripts　that　are　involved　in　a　broad　range　of　biological　processes.　Here,　we　examine　the　functional　role　of　lncRNAs　in　feather　regeneration.　RNA-seq　profiling　of　the　regenerating　feather　blastema　revealed　that　Wnt　signaling　is　among　the　most　active　pathways　during　feather　regeneration,　with　Wnt　ligands　and　their　inhibitors　showing　distinct　expression　patterns.　Co-expression　analysis　identified　hundreds　of　lncRNAs　with　similar　expression　patterns　to　either　the　Wnt　ligands　(the　Lwnt　group)　or　their　downstream　target　genes　(the　Twnt　group).　Among　these,　we　randomly　picked　two　lncRNAs　in　the　Lwnt　group　and　three　lncRNAs　in　the　Twnt　group　to　validate　their　expression　and　function.　Members　in　the　Twnt　group　regulated　feather　regeneration　and　axis　formation,　whereas　members　in　the　Lwnt　group　showed　no　obvious　phenotype.　Further　analysis　confirmed　that　the　three　Twnt　group　members　inhibit　Wnt　signal　transduction　and,　at　the　same　time,　are　downstream　target　genes　of　this　pathway.　Our　results　suggest　that　the　feather　regeneration　model　can　be　utilized　to　systematically　annotate　the　functions　of　lncRNAs　in　the　chicken　genome.　©　2018.　Published　by　The　Company　of　Biologists　Ltd.