Dendritic　mesoporous　silica　microspheres　were　prepared　by　sol-gel　method　with　ammonium　as　catalyst,　TEOS　as　silica　source　and　CTAB　as　surfactant.　Nano　Ag　was　in-situ　loaded　in　the　SiO　2　microspheres.　The　effect　of　different　amounts　of　ether　and　silane　structure　promoter　on　the　morphology,　structure　and　particle　size　of　dendritic　mesoporous　SiO　2　microspheres　was　studied.　The　minimum　inhibitory　concentration　(MIC)　and　minimum　bactericidal　concentration　(MBC)　of　the　samples　were　tested　by　antibacterial　examination.　The　results　show　that　the　addition　of　silane　structure　promoter　and　ether　is　crucial　to　the　formation　of　dendritic　pores.　The　MIC　of　nano　Ag　in　the　mesoporous　silica　is　between　3.16mg•L　-1　and　3.95mg•L　-1　,　the　MBC　is　6.32mg•L　-1　.　Both　of　them　are　superior　to　pure　nano　Ag　in　antibacterial　properties.　©　2018,　Journal　of　Materials　Engineering.　All　right　reserved.