Light-Induced Activation of c-Met Signalling by Photocontrolled DNA Assembly - Details

author：

Chen, S. (Chen, S..) ^[1] | Li, J. (Li, J..) ^[2] | Liang, H. (Liang, H..) ^[3] | Lin, X.-H. (Lin, X.-H..) ^[4] | Yang, H.-H. (Yang, H.-H..) ^[6]

Indexed by：

Scopus

Abstract：

Optical　manipulation　appears　to　be　a　powerful　tool　for　spatiotemporally　controlling　a　variety　of　cellular　functions.　Herein,　a　photocontrolled　DNA　assembly　approach　is　described　which　enables　light-induced　activation　of　cellular　signal　transduction　by　triggering　protein　dimerization　(c-Met　signalling　in　this　case).　Three　kinds　of　DNA　probes　are　designed,　including　a　pair　of　receptor　recognition　probes　with　adaptors　and　a　blocker　probe　with　a　photocleavable　linker　(PC-linker).　By　implementing　PC-linkers　in　blocker　probes,　the　designed　DNA　probes　response　to　light　irradiation,　which　then　induces　the　assembly　of　receptor　recognition　probes　through　adaptor　complementing.　Consequently,　light-mediated　DNA　assembly　promotes　the　dimerization　of　c-Met　receptors,　resulting　in　activation　of　c-Met　signalling.　It　is　demonstrated　that　the　proposed　photocontrolled　DNA　assembly　approach　is　effective　for　regulating　c-Met　signalling　and　modulating　cellular　behaviours,　such　as　cell　proliferation　and　migration.　Therefore,　this　simple　approach　may　offer　a　promising　strategy　to　manipulate　cell　signalling　pathways　precisely　in　living　cells.　©　2018　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim

Keyword：

aptamers; DNA assembly; light-induced; living cells; signal transduction

Community：

[ 1 ] [Chen, S.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 2 ] [Li, J.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Liang, H.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 4 ] [Lin, X.-H.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 5 ] [Li, J.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 6 ] [Yang, H.-H.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350116, China

Reprint 's Address：

[Li, J.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou UniversityChina

Email：

lijuan@fzu.edu.cn

Show more details

Related Keywords：

Source ：

Chemistry - A European Journal

ISSN： 0947-6539

Year： 2018

Issue： 60

Volume： 24

Page： 15988-15992

5 . 1 6

JCR@2018

3 . 9 0 0

JCR@2023

ESI HC Threshold：209

JCR Journal Grade：1

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 31

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 4

Affiliated Colleges：

Get Fulltext

DOI Library Discovery Baidu Scholar Search SCOPUS

Type
Departments

All Years Choose Year From to