Flow injection and liquid chromatographic detection of amino acids based on the chemiluminescence reaction of lucigenin - Details

author：

Chen, G.N. (Chen, G.N..) ^[1] | Xu, X.Q. (Xu, X.Q..) ^[2] | Duan, J.P. (Duan, J.P..) ^[3] | Lin, R.E. (Lin, R.E..) ^[4] | Zhang, F. (Zhang, F..) ^[5]

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Abstract：

It　has　been　found　that　amino　acids　can　inhibit　the　chemiluminescent　reaction　of　lucigenin,　especially　in　the　presence　of　CoII,　and　that　the　extent　of　inhibition　is　proportional　to　the　concentration　of　the　amino　acids.　Based　on　this　principle,　flow　injection　(FI)　and　LC　methods　have　been　developed　to　determine　amino　acids.　The　linear　ranges　for　sixteen　amino　acids　were　determined　in　the　FI　system,　and　the　retention　times　of　eleven　amino　acids　were　investigated　by　LC,　and　a　mixture　of　Asp,　Val,　Leu　and　Phe　was　analysed.　The　method　is　simple　and　rapid,　with　a　time　for　one　analysis　of　less　than　10　min.

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[ 1 ] [Chen, G.N.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 2 ] [Xu, X.Q.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 3 ] [Duan, J.P.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 4 ] [Lin, R.E.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 5 ] [Zhang, F.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China

Reprint 's Address：

[Chen, G.N.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China

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Source ：

Analytical Communications

ISSN： 1359-7337

Year： 1996

Issue： 3

Volume： 33

Page： 99-102

2 . 3 7 1

JCR@2001

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