N-(β-Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay - Details

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author：

Zhuang, H.-S. (Zhuang, H.-S..) ^[1] | Wang, Q.-E. (Wang, Q.-E..) ^[2] | Zhang, F. (Zhang, F..) ^[3] | Huang, Q.-J. (Huang, Q.-J..) ^[4] | Tang, Y.-C. (Tang, Y.-C..) ^[5]

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Abstract：

A　new　chemiluminesceuce　label　N-(β-carboxypropionyl)luminol　(CPL)　was　used　to　label　sheep　anti-human　IgG　(SaHIgG).　The　labeled　antibody　was　stable　and　could　be　detected　at　least　down　to　10-17　∼10-16　mol.　The　molar　incorporation　ratio　was　estimated　to　be　0.26　mol　of　CPL　per　mol　of　SaHIgG.　There　were　no　apparent　changes　in　the　immunoreactivity　of　the　labeled　SaHIgG　and　in　the　quantum　efficiency　of　the　CPL　after　labeling.

Keyword：

Chemiluminescence immunoassay; N-(β-carboxypropionyl)luminol; Sheep anti-human IgG

Community：

[ 1 ] [Zhuang, H.-S.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 2 ] [Wang, Q.-E.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 3 ] [Zhang, F.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 4 ] [Huang, Q.-J.]Fuzhou General Hospital, Nanjing Military Region, Fuzhou, Fujian 350002, China
[ 5 ] [Tang, Y.-C.]Fuzhou General Hospital, Nanjing Military Region, Fuzhou, Fujian 350002, China

Reprint 's Address：

[Zhuang, H.-S.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

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Source ：

Chinese Journal of Chemistry

ISSN： 1001-604X

Year： 1997

Issue： 2

Volume： 15

Page： 123-129

0 . 2 5 7

JCR@1997

5 . 5 0 0

JCR@2023

JCR Journal Grade：4

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 0

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