A　method　of　calculation　of　the　amount　of　pyrene　absorbed　from　the　fluorescence　emission　spectrum　has　been　developed.　By　this　method　the　solubilization　of　pyrene　from　H2O/DMF　solvent　mixture　to　the　crew-cut　micelles　of　polystyrene-b-polyacrylic　acid　diblock　copolymer　has　been　investigated.　The　results　showed　that　the　solubilization　of　pyrene　depends　strongly　upon　water　content　in　the　solvent　mixture.　The　amount　of　pyrene　absorbed　was　enhanced　with　increasing　the　water　content.　The　absorption　isotherms　were　calculated　and　discussed　by　combination　of　the　formula　developed　and　the　partition　coefficient　Kv.　The　theoretical　treatment　suggested　an　approach　to　calculate　Kv　directly　from　the　intensity　values　measured　experimentally.