The　concentration　of　rutin　and　its　hydrolysis　products　were　directly　minitored　by　capillary　electrophoresis-amperometric　detection　due　to　its　high　resolution　and　high　sensitivity　of　detection.　The　hydrolysis　rate　constants　of　rutin　can　be　obtained　at　different　temperatures　in　10%　HCl.　At　65°C,　80°C　and　95°C,　the　measured　rate　constants　of　rutin　hydrolysis　were　5.　66　×　10-3　min-1,　7.19　×　10-3　min-1　and　8.51　×　10-3　min-1,　respectively.　The　method　was　intuitionistic　and　convenient.　The　approach　can　also　be　used　for　separation　and　determination　of　the　rutin　and　quercetin.　Under　the　optimum　conditions,　the　detection　limit　was　0.6　and　6.2　mg/L　for　rutin　and　quercetin,　respectively.　This　method　was　successfully　applied　to　the　analysis　of　traditional　medicine　Huaimi　sample　with　average　recoveries　of　97.6%.