A　strain　Aspergillus　spp.　F1　which　could　effectively　metabolized　omethoate　was　screened　out　in　this　study.　F1　tended　to　form　granula　with　diameter　4-5　mm　after　5　days　culture　in　shaker.　The　pH　range　from　4.5　to　6.5　was　the　suitable　pH　range　for　growth　and　metabolism　of　Aspergillus　spp.　F1.　The　maximum　omethoate　removal　rate　was　about　3.0mg/(hL),　and　the　removal　fraction　of　omethoate　reached　90%　after　8　days　culture　when　initial　concentrations　of　omethoate　were　not　more　than　2000　mg/L　in　medium.　There　was　no　obvious　relativity　between　cell　growth　and　cometabolism　of　omethoate.　Starch　was　the　best　carbon　source　for　omethoate　removal　and　the　result　after　3　days　reached　56.6%　removal.　F1　could　use　omethoate　to　metabolize　as　single　nitrogen　or　phosphate　source.　The　residual　fragments　in　medium　after　treatment　with　the　Aspergillus　spp.　F1　were　determined　by　gas　chromatography-mass　spectrometry.　The　analysis　results　showed　that　only　fragment　o,o,s,-trimethyl　phosphorothioate　(TEP)　containing　phosphorus　was　available　and　the　fragment　containing　nitrogen　was　consumed　by　F1　thoroughly　in　culture　process.　But　no　accumulations　of　TEP　were　observed　in　the　omethoate　bioremediation　process.　F1　could　effectively　remove　omethoate　in　complex　nutritional　environment　safely.　©　2004　Elsevier　Ltd.　All　rights　reserved.