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author:

Chen, T. (Chen, T..) [1] | Orr, D.F. (Orr, D.F..) [2] | O'Rourke, M. (O'Rourke, M..) [3] | McLynn, C. (McLynn, C..) [4] | Bjourson, A.J. (Bjourson, A.J..) [5] | McClean, S. (McClean, S..) [6] | Hirst, D. (Hirst, D..) [7] | Rao, P. (Rao, P..) [8] | Shaw, C. (Shaw, C..) [9]

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Scopus

Abstract:

Tryptophyllins are a heterogenous group of amphibian skin peptides originally identified in skin extracts of Neotropical leaf frogs, Phyllomedusa sp., by chemical means. Until now, biosynthetic precursor structure and biological activity remain unreported. Here we describe the isolation of a novel, post-translationally modified tryptophyllin, Lys-Pro-Hyp-Ala-Trp-Val-Pro. amide (PdT-1), from the skin secretion of the Mexican leaf frog, Pachymedusa dacnicolor. Using a 3′- and 5′-RACE strategy and an in vitro skin cDNA library, the PdT-1-encoding precursor was cloned and found to consist of an open-reading frame of 62 amino acids with a single copy of PdT-1 located towards the C-terminus. A synthetic replicate of PdT-1 was found to be a potent myoactive agent, relaxing mammalian arterial smooth muscle and contracting small intestinal smooth muscle at nanomolar concentrations. PdT-1 is thus the first amphibian skin tryptophyllin to be pharmacologically characterized and the first whose precursor cDNA has been cloned. © 2003 Elsevier B.V. All rights reserved.

Keyword:

Amphibian; cDNA clone; Pachymedusa dacnicolor; Peptide; Pharmacological activity

Community:

  • [ 1 ] [Chen, T.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 2 ] [Chen, T.]Institute of Biotechnology, Fuzhou University, Fuzhou 350002, Fujian Province, China
  • [ 3 ] [Orr, D.F.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 4 ] [O'Rourke, M.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 5 ] [McLynn, C.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 6 ] [Bjourson, A.J.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 7 ] [McClean, S.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 8 ] [Hirst, D.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom
  • [ 9 ] [Rao, P.]Institute of Biotechnology, Fuzhou University, Fuzhou 350002, Fujian Province, China
  • [ 10 ] [Shaw, C.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom

Reprint 's Address:

  • [Shaw, C.]School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, United Kingdom

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Source :

Regulatory Peptides

ISSN: 0167-0115

Year: 2004

Issue: 1

Volume: 117

Page: 25-32

2 . 5 3 1

JCR@2004

1 . 8 1 3

JCR@2015

JCR Journal Grade:2

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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