An　anion　exchange　chromatographic　column　(DEAE-650C)　and　a　cation　exchange　chromatographic　column　(CM-650C)　were　connected　in　series　on　a　perfusion　chromatography　workstation.　The　crude　extract　of　bitter　melon　seeds　flowed　through　the　two　columns　and　the　unadsorbed　fraction　on　the　DEAE-650C　column　was　then　directly　readsorbed　on　the　CM-650C　column.　Two　protein　components　with　antifungal　activity　were　eluted　from　the　cation　exchange　chromatographic　column　by　linear　salt　gradient.　Both　of　them　were　found　to　be　homogeneous　by　sodium　dodecyl　sulfate　polyacrylamide　gel　electrophoresis　(SDS-PAGE),　and　their　relative　molecular　masses　were　estimated　to　be　about　30　000.　Their　N-terminal　amino　acid　sequences　are　DVSFRLSGADPRSYGMFI　and　DVNFDLSTATAK.　All　of　the　above　suggested　that　they　are　α-momorcharin　(α-MMC)　and　β-momorcharin　(β-MMC),　respectively,　two　type　I　ribosome-inactivating　proteins　(Rips)　of　bitter　melon　seeds.　α-MMC　shows　antifungal　activity　against　Fusarium　oxysporum　and　Pythium　aphanidermatum,　while　β-MMC　shows　antifungal　activity　against　Pythium　aphanidermatum.　But　they　are　not　against　Sclerotium　rolfsii.　In　the　study,　quantitative　recoveries　of　α-MMC　and　β-MMC　were　13.　8%　and　8.　0%,　respectively,　from　decorticated　seeds　by　ion　exchange　chromatographic　columns　in　series.