Digital　microfluidic　biochips　(DMFBs)　play　an　important　role　in　the　biochemical　industry　due　to　their　low　cost,　portability　and　high　efficiency.　It＇s　reported　that　the　biochip　growth　is　twice　as　large　as　before　in　the　market.　However,　the　resolution　of　microfluidic　biochip　security　has　not　kept　pace　with　the　development　of　business　opportunities,　and　there　are　still　many　major　problems.　One　of　the　problems　is　the　hardware　Trojans.　According　to　the　classification　of　hardware　Trojans　based　on　biochips,　in　general-purpose　DMFBs,　DMFB　is　configured　to　execute　specific　biochemical　protocols.　Attackers　can　modify　these　protocols　by　changing　the　actuation　sequences　in　the　field.　In　this　paper,　due　to　the　cyberphysical　microfluidic　biochips,　we　consider　using　an　efficient　protect　model,　based　on　Hamming　distance,　to　detect　differences　between　two　sequences.　This　protect　model　can　be　used　to　find　out　if　the　actuation　sequence　has　been　tampered　with.　It　is　simple,　effective　and　it　has　a　low　error　rate.　The　experimental　results　prove　the　feasibility　of　our　proposed　method.　©　2020　IEEE.