This　paper　aims　to　perform　an　objective　quantitative　analysis　on　quadriceps　rehabilitation,　and　investigate　the　muscle　status　in　cross-over　design　under　rehabilitation　actions.　Rehabilitation　actions　are　modeled　using　the　human　body　modeling　software　AnyBody,　which　can　analyze　the　variations　in　the　muscle　activity　and　muscle　force　of　the　quadriceps　femoris　during　thigh　flexion.　In　addition,　it　can　experimentally　validate　the　effectiveness　of　the　model　in　combination　with　electromyographic　(EMG)　signals　of　three　quadriceps　femoris　muscles　during　different　activities.　According　to　the　study　results,　the　rehabilitation　actions　of　the　quadriceps　femoris　can　be　quantified　by　means　of　collecting　EMG　signals.　©　2020　Published　under　licence　by　IOP　Publishing　Ltd.