In　this　paper,　an　ambient　vibration-based　seismic　response　analysis　procedure　of　an　existing　large-span　prestressed　concrete　continuous　rigid-framed　bridge　is　proposed.　The　procedure　includes　field　ambient　vibration　testing,　finite　element　(FE)　modeling,　parameter　calibration　of　FE　model,　selection　of　earthquake　ground　motion,　and　dynamic　time-history　analysis　of　the　bridge,　et　al..　Based　on　this　procedure,　the　seismic　response　analysis　of　a　5-span　prestressed　concrete　continuous　rigid-framed　bridge,　namely　the　Quanzhou　Houzhu　Bridge,　was　conducted.　It　is　demonstrated　that　the　vertical　earthquake　ground　motion　and　the　traveling　wave　effects　should　be　considered　during　the　seismic　response　analysis　of　the　continuous　rigid-framed　bridge.　The　results　also　reveal　that　the　largest　seismic　response　inner-forces　occur　at　the　bottom　of　the　piers　and　the　connection　between　the　box-girder　and　the　pier.　This　kind　of　analysis　can　serve　for　the　long-term　health　monitoring,　maintenance　and　rehabilitation　and　it　has　great　importance.