The　adsorption　equilibrium　and　kinetics　of　Sisomicin　on　macroporous　weakly　basic　acid　resin　were　studied.　The　surface　diffusion　model　was　established.　The　intraparticle　surface　diffusion　coefficient　and　mass　transfer　coefficient　were　obtained　by　regression　of　the　experiment　data　of　adsorption　rate　curves　compared　with　orthogonal　collocation　method　and　genetic　algorithms　directly.　The　mean　square　errors　were　not　more　than　0.025.　The　results　showed　that　the　numerical　solutions　agreed　with　the　experiment　data　of　adsorption　rate　curves.　The　adsorption　equilibrium　of　Sisomicin　on　macroporous　weakly　basic　acid　resin　D113　was　in　accord　with　Langmuir　equation.　The　adsorption　process　on　D113　could　be　described　with　the　surface　diffusion　model　very　well.　The　intraparticle　effective　diffusion　coefficient　was　determined　to　be　2.00　×　10-8-4.09　×　10-8　cm2/s　in　experimental　condition.　The　internal　mass　transfer　coefficient　was　9.00　×　10-5　cm/s-2.95　×　10-4　cm/s.