Non-thermal　pasteurization　processes　can　offer　higher　quality,　better　nutrition　retention　and　more　energy　efficient　compared　with　traditional　thermal　processes.　In　this　paper,　dielectric　barrier　discharge　reactor　(DBDR)　was　designed.　And　the　bactericidal　efficiency　of　low-temperature　plasma　(LTP)　produced　by　DBDR　on　fresh　juice　and　its　impacts　on　the　quality　of　juice　were　researched.　The　second　order　quadratic　equation　for　DBDR　inactivation　was　established　by　response　surface　methodology,　and　the　effectiveness　of　this　model　was　verified.　Also,　effects　of　cycles,　pH　value,　temperature　and　voltage　on　DBDP　sterilization　of　E.coli　were　discussed.　Based　on　the　exploration,　the　optimum　conditions　for　inactivation　of　E.coli　ATCC　8739　of　seven　log　cycles　were　obtained　as:　6　cycles,　pH　3.14,　temperature　39.72°C,　and　voltage　21.42　kV.　It　lays　a　foundation　for　further　studying　on　the　DBDP　sterilization　law　and　the　mechanism　of　sterilization.　While　the　correlation　coefficient　R2　for　this　model　equals　to　0.9817,　the　low-temperature　plasma　(LTP)　produced　by　DBDR　can　be　applied　to　other　fruit　and　vegetable　disinfection　treatment　and　improve　the　quality　and　safty　of　fresh　fruit　and　vegetable.