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[期刊论文]

Optimization of tea dregs protein complexes limited enzymatic hydrolysis and application in liposome-meat system

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author:

Zhao, Lina (Zhao, Lina.) [1] | Wang, Shaoyun (Wang, Shaoyun.) [2] | Jiang, Yong (Jiang, Yong.) [3] | Unfold

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Abstract:

Objective: This paper aims to study tea dregs protein complexes limited enzymatic hydrolysis and antioxidant activity in liposome-meat system, which can provide a theoretical basis and experimental evidence for further research of activity and antioxidation mechanism in tea dregs protein pep peptide complexes. Methods: Tea dregs protein was limited enzymatic hydrolysis by complex protease, then to study antioxidant activity of the peptide complex in liposome-meat system and compare with the traditional synthetic antioxidants VC, BHT was engaged. Tea dregs protein peptide complexes were separated by Nanofiltration tubular membrane system to study the relationship between molecular weight and antioxidant activity. Results: The technology of limited enzymatic hydrolysis was selected as temperature 50°C, pH 7.0, ratio of protease to substrate 6 000 U/g, substrate concentration 1.5%, hydrolysis time 20 min. The DH of tea dregs protein after limited hydrolysis was determined to be 9%. The antioxidant activity was more than VC, but less than BHT in LA system, and it had strong antioxidant activity in chicken products. The antioxidant activity of peptide complexes with molecular weight above 8 000 Da was stronger than that of other components. Conclusion: With strong antioxidant activity, tea dregs protein peptide complex has the potential to replace synthetic antioxidants.

Keyword:

Antioxidants Enzymatic hydrolysis Meats Molecular weight Peptides

Community:

  • [ 1 ] [Zhao, Lina]College of Biological Science and Engineering, Fuzhou University, Fuzhou 350002, China
  • [ 2 ] [Wang, Shaoyun]College of Biological Science and Engineering, Fuzhou University, Fuzhou 350002, China
  • [ 3 ] [Jiang, Yong]Fujian Xian Yangyang Food and Technology Co. Ltd., Ningde 352101, Fujian, China
  • [ 4 ] [Zhou, Shaoqian]Fujian Xian Yangyang Food and Technology Co. Ltd., Ningde 352101, Fujian, China
  • [ 5 ] [Huang, Jiachi]Fujian Xian Yangyang Food and Technology Co. Ltd., Ningde 352101, Fujian, China

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Source :

Journal of Chinese Institute of Food Science and Technology

ISSN: 1009-7848

CN: 11-4528/TS

Year: 2013

Issue: 5

Volume: 13

Page: 92-99

Cited Count:

WoS CC Cited Count:

30 Days PV: 3

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