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Objective: This paper aims to study tea dregs protein complexes limited enzymatic hydrolysis and antioxidant activity in liposome-meat system, which can provide a theoretical basis and experimental evidence for further research of activity and antioxidation mechanism in tea dregs protein pep peptide complexes. Methods: Tea dregs protein was limited enzymatic hydrolysis by complex protease, then to study antioxidant activity of the peptide complex in liposome-meat system and compare with the traditional synthetic antioxidants VC, BHT was engaged. Tea dregs protein peptide complexes were separated by Nanofiltration tubular membrane system to study the relationship between molecular weight and antioxidant activity. Results: The technology of limited enzymatic hydrolysis was selected as temperature 50°C, pH 7.0, ratio of protease to substrate 6 000 U/g, substrate concentration 1.5%, hydrolysis time 20 min. The DH of tea dregs protein after limited hydrolysis was determined to be 9%. The antioxidant activity was more than VC, but less than BHT in LA system, and it had strong antioxidant activity in chicken products. The antioxidant activity of peptide complexes with molecular weight above 8 000 Da was stronger than that of other components. Conclusion: With strong antioxidant activity, tea dregs protein peptide complex has the potential to replace synthetic antioxidants.
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Journal of Chinese Institute of Food Science and Technology
ISSN: 1009-7848
CN: 11-4528/TS
Year: 2013
Issue: 5
Volume: 13
Page: 92-99
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