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author:

Zhu, Xi (Zhu, Xi.) [1] | Zheng, Hanye (Zheng, Hanye.) [2] | Xu, Huifeng (Xu, Huifeng.) [3] | Lin, Ruolan (Lin, Ruolan.) [4] | Han, Yejian (Han, Yejian.) [5] | Yang, Guidi (Yang, Guidi.) [6] | Lin, Zhenyu (Lin, Zhenyu.) [7] | Guo, Longhua (Guo, Longhua.) [8] | Qiu, Bin (Qiu, Bin.) [9] | Chen, Guonan (Chen, Guonan.) [10]

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EI

Abstract:

In this work, a portable immunosensor was developed using a personal glucose meter (PGM) as the signal transducer. In this system, the anti-alpha-fetoprotein (anti-AFP) is immobilized on the screen-printed gold electrode (SPGE) surface. The presence of a target brought in the fixation of invertase-anti-AFP on anti-AFP modified SPGE; then, invertase catalyzed sucrose to glucose, which was detected using the PGM. The reading of the PGM exhibited a linear relationship with the AFP concentration. The detection limit is 0.18 ng mL-1. After one test, this immunosensor can be reused at least 5 times using an easy treatment. Moreover, the assay results of the clinical serum samples are satisfactory. © 2014 The Royal Society of Chemistry.

Keyword:

Glucose Immunosensors Transducers

Community:

  • [ 1 ] [Zhu, Xi]College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • [ 2 ] [Zhu, Xi]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 3 ] [Zheng, Hanye]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 4 ] [Xu, Huifeng]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 5 ] [Xu, Huifeng]Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou Fujian, China
  • [ 6 ] [Lin, Ruolan]College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • [ 7 ] [Han, Yejian]College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • [ 8 ] [Yang, Guidi]College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China
  • [ 9 ] [Lin, Zhenyu]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 10 ] [Guo, Longhua]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 11 ] [Qiu, Bin]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China
  • [ 12 ] [Chen, Guonan]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China

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Source :

Analytical Methods

ISSN: 1759-9660

Year: 2014

Issue: 14

Volume: 6

Page: 5264-5268

1 . 8 2 1

JCR@2014

2 . 7 0 0

JCR@2023

ESI HC Threshold:268

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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