The　objective　of　this　study　was　to　understand　and　optimize　the　formation　of　microalgae　biofilms　in　specific　culture　conditions.　Firstly,　the　adhesion　of　six　freshwater　algae　species　was　compared.　Chlorococcum　sp.　was　selected　because　of　the　high　adhesion　biomass　productivity　(ABP)　and　adhesion　rate　achieved.　Secondly,　the　adhesion　of　Chlorococcum　sp.　was　compared　with　nine　commonly　used　supporting　materials,　and　glass　fiber-reinforced　plastic　proved　to　be　the　optimal　substrata.　Thirdly,　based　on　response　surface　methodology　experiments,　a　second-order　polynomial　model　was　developed　to　examine　the　effect　of　culture　period,　initial　total　nitrogen　concentration　(ITNC)　in　manure　wastewater,　pH　and　culture　volume　of　the　growth　chamber　on　the　adhesion　of　Chlorococcum　sp.　using　glass　fiber-reinforced　plastic.　The　experimental　and　modeling　results　showed　that　ITNC,　pH　and　culture　volume　as　well　as　the　interactions　between　culture　period　and　ITNC,　culture　period　and　culture　volume　were　significant　on　ABP.　Optimum　culture　conditions　were　predicted　at　a　culture　period　of　11　days,　ITNC　of　70　mg　L-1,　pH　of　8　and　culture　volume　of　340　mL,　under　which　the　predicted　maximum　ABP　was　4.26　g　m-2　day-1.　The　prediction　was　close　to　validation　experimental　results,　indicating　that　the　model　could　be　used　to　guide　and　optimize　the　attached　culture　of　Chlorococcum　sp.　using　glass　fiber-reinforced　plastic.　©　2013　Springer-Verlag.