Chrysoidin　is　a　kind　of　banned　food　dye,　and　it　has　been　illegally　used　for　coloring　food.　A　rapid　detection　and　quantification　method　is　developed　and　applied　in　analysis　chrysoidin　in　yuba.　Gold　nanoparticles　are　synthesized　by　using　hexadecyl　trimethyl　ammonium　bromide　(CTAB)　as　the　bifunctional　ligand　to　link　the　solid　substrate　and　the　AuNPs.　The　laser　wavelength　used　for　quantitative　is　1　594　cm-1.　Significant　differences　between　different　concentrations　of　chrysoidin　are　verified　by　multiple　variable　analysis.　A　relationship　between　the　logarithm　of　the　concentrations　and　the　intensity　of　laser　is　proved　using　univariate　analysis　method.　The　calibration　curves　showed　good　linearity　in　the　range　of　0.001~0.5　mmol·L-1　with　correlation　coefficients　r=0.995.　The　method　is　successfully　applied　to　the　determination　of　chrysoidin　in　yuba.　The　average　recoveries　of　the　drugs　spiked　at　50　and　500　μg·g-1　levels　are　82.4%　and　116.9%,　and　the　relative　standard　deviations　(RSD)　are　3.8%　and　4.0%.　The　method　is　simple,　rapid,　sensitive　and　accurate　in　the　determination　of　chrysoidin.　©　2015,　Science　Press.　All　right　reserved.