Objective:　Lipase-producing　marine　microorganisms　were　isolated　and　identified,　and　the　enzymatic　properties　were　studied.　Methods:　Lipase-producing　marine　microorganisms　were　isolated　with　selective　mediacontained　olive　oil　as　the　only　source　of　carbon.　The　objective　strain　was　identified　according　to　the　sequence　analysis　of　16S　rDNA,　and　the　enzymatic　properties　were　studied.　Results:　Twenty-twolipase-producing　marine　microorganisms　were　isolated　from　five　samples,　such　as　the　intestinal　contents　of　eeland　soil　samples.　The　lipase　produced　by　strain　ZF-16　had　the　highest　activity　to　catalyze　glycerolysis　of　oil.　Strain　ZF-16　was　identified　as　Burkholderiacepacia　according　to　its　morphology　characteristics　and　the　sequence　of　16S　rDNA.　The　optimal　pH　and　temperature　of　the　lipase　were　pH　9.0　and　75,　respectively.　Activity　of　lipase　was　relatively　stable　at　40　and　pH　5.0-10.0.　Besides,　the　activity　of　lipase　wasactivated　by　1　mmol/L　Na+　and　K+,　and　strongly　inhibited　by　Zn2+.　The　lipase　could　degrade　substrates　with　different　chain　length　from　C4　to　C18,　especially　tricaprylin　(C8).　Conclusion:　A　lipase-producing　marine　microorganism　was　successfully　isolated　and　identified　as　Burkholderiacepacia.　The　results　showed　that　the　lipase　was　a　kind　of　alkaline　lipases　resisting　to　high　temperature,　and　had　degradation　effects　on　substrates　with　different　chain　length　from　C4　to　C18.　Therefore,　this　lipase　could　be　used　in　food　baking,　pulp　and　paper　industryand　waste-paper　deinking.　©　2015,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.