Background:　CO2　emission,water　pollution　and　petroleumshortage　are　the　issues　comingwith　the　development　of　industry.　A　cost　effective　system　was　constructed　to　fix　the　CO2　in　flue　gas　(15%　CO2),　remove　nitrogen　and　phosphorus　from　manure　wastewater　and　produce　biofuels　at　the　same　time.　The　significant　cultivation　conditions　were　selected　by　Plackett–Burman　design,　and　then　optimized　with　central　composite　design.　Results:　Optimum　culture　condition　was　predicted　at　light　intensity　of　238　μmol·m-2·s-1,　TN　of　152　mg·L-1,　inoculum　density　of　0.3　g·L-1,　under　which　the　measured　CO2　fixation　rate,　total　nitrogen　and　phosphorus　removing　rate,　and　lipid　content　were　638.13　mg·L-1·d-1,　88.16%,　73.98%　and　11.9%,　respectively.　The　lipid　content　was　then　enhanced　to　24.2%　by　a　nitrogen　starvation　strategy.　Conclusion:　A　cultivation　strategy　was　suggested　to　achieve　effective　C/N/P　removal　from　flue　gas　and　manure　wastewater,　and　meanwhile　obtained　high　lipid　content　from　microalgal　biomass.　©　2014　Pontificia　Universidad　Católica　de　Valparaíso.　Production　and　hosting　by　Elsevier　B.V.　All　rights　reserved.