An　enzyme-based　method　for　destaining　polyacrylamide　gels　stained　with　Coomassie　Brilliant　Blue　R-250　is　described.　Distilled　water　supplemented　with　diluted　fermentation　broth　of　a　laccase-producing　white-rot　fungus,　Cerrena　sp.,　was　used　for　gel　destaining,　and　a　clear　gel　background　was　obtained　in　2　h　at　37　°C.　Sensitivity　of　protein　detection　was　10　ng.　The　method　did　not　require　organic　solvents　or　changing　the　destaining　solution.　Due　to　simultaneous　gel　destaining　and　dye　decolorization,　the　colorless　destaining　solution　can　be　disposed　of　directly.　Laccase　destaining　of　polyacrylamide　gels　was　simple,　efficient,　and　environmentally　friendly.　©　2015　Elsevier　Inc.　All　rights　reserved.