The　low-cost　large-scale　fabrication　of　microlens　arrays　(MLAs)　with　precise　alignment,　great　uniformity　of　focusing,　and　good　converging　performance　are　of　great　importance　for　integral　imaging　3D　display.　In　this　work,　a　simple　and　effective　method　for　large-scale　polymer　microlens　arrays　using　screen　printing　has　been　successfully　presented.　The　results　show　that　the　MLAs　possess　high-quality　surface　morphology　and　excellent　optical　performances.　Furthermore,　the　microlens＇　shape　and　size,　i.e.,　the　diameter,　the　height,　and　the　distance　between　two　adjacent　microlenses　of　the　MLAs　can　be　easily　controlled　by　modifying　the　reflowing　time　and　the　size　of　open　apertures　of　the　screen.　MLAs　with　the　neighboring　microlenses　almost　tangent　can　be　achieved　under　suitable　size　of　open　apertures　of　the　screen　and　reflowing　time,　which　can　remarkably　reduce　the　color　moiré　patterns　caused　by　the　stray　light　between　the　blank　areas　of　the　MLAs　in　the　integral　imaging　3D　display　system,　exhibiting　much　better　reconstruction　performance.　©　2016　American　Chemical　Society.