In　this　paper,　coupled　with　hybridization　chain　reaction　and　portable　personal　glucose　meter,　a　highly　sensitive　aptamer-based　immunoassay　for　vascular　endothelial　growth　factor　(VEGF)　is　fabricated.　The　antibody　immobilized　on　the　microplate　captured　the　target　protein,　which　would　be　sandwiched　by　the　formation　of　aptamer/VEGF/antibody　complex　in　the　presence　of　aptamer.　And　then,　an　18-nt　elongated　strand　at　the　3′　end　of　aptamer,　with　the　assist　of　invertase-functionalized　auxiliary　probe,　triggered　the　hybridization　chain　reaction　to　generate　invertase-concatamers.　Vast　invertase　catalyzed　sucrose　to　glucose,　which　is　monitored　through　personal　glucose　meter.　Finally,　this　portable　sensing　system　can　achieve　the　detection　of　limit　of　1.2 pg/mL　with　good　stability　and　selectivity.　Meanwhile,　VEGF　in　the　serum　would　also　be　assayed　with　satisfied　result.　©　2017