A　chitinase-producing　strain　B120　was　isolated　from　Fujian　soil　and　identified　as　Bacillus　cereus　by　16S　rDNA　sequence　analysis.　The　chitinase-producing　media　were　optimized　and　the　optimal　medium　formulations　were　ob　tained　and　as　follow:　lactose　3.5%,　peptone　1.0%,　powdered　chitin　0.2%,　K2HPO4　0.07%,　KH2PO4　0.03%,　MgSO4　0.05%,　FeSO4-H20　0.002%,　NaCl　0.5%,　pH　6.0.　Fermenting　under　these　conditions　for　84　h,　chitinase　activity　could　reach　603　U/L,　5.93　times　higher　than　before　optimization　(87　U/L).　Furthermore,　the　chitinase　properties　were　studied.　The　results　showed　that　the　optimum　reaction　pH　and　temperature　were　8.0　and　50,　respectively.　This　enzyme　was　stable　under　30　and　pH　7.0-10.0,　and　more　than　74%　of　relative　activity　was　retained　after　incubation　for　1　h.　It　was　a　kind　of　mesothermal　and　alkaline　chitinases.　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.