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author:

Lin, Mengdan (Lin, Mengdan.) [1] | Wang, Guozeng (Wang, Guozeng.) [2] (Scholars:王国增) | X., Ye (X., Ye.) [3] (Scholars:叶秀云) | J., Lin (J., Lin.) [4] (Scholars:林娟)

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EI Scopus PKU CSCD

Abstract:

Strain 825 with α-amylase activity was isolated from marine samples of Pingtan sea and Tai wan strait and identified as Bacillus sp. by 16S rDNA sequence analysis. Amylase-producing medium of strain 825 was optimized, and the optimum conditions were as follow: soluble starch 2.0%, yeast extract 0.5%, peptone 1.5%, initial pH7.0, fermentation period 60 h. Under these conditions, α-amylase activity increased from 46.55 U/mL to 77.44 U/mL, 66.36% higher than before. Furthermore, the α-amylase properties were analyzed and the results were obtained. The optimal reaction temperature of α-amylase was 70, and more than 90% of its maximum activity was maintained after incubation for 1 h under 30-50. The optimal reaction pH was 7.0, and more than 70% activity was retained after incubation for 1 h in the pH 5.0-9.0. It indicated that this α-amylase had a wide pH tolerance range. © 2017, Editorial Office of Journal of CIFST. All right reserved.

Keyword:

Amylases Bacteriology Fermentation Microorganisms Starch

Community:

  • [ 1 ] [Lin, Mengdan]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350116, China
  • [ 2 ] [Wang, Guozeng]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350116, China
  • [ 3 ] [Wang, Guozeng]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou; 350116, China

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Source :

Journal of Chinese Institute of Food Science and Technology

ISSN: 1009-7848

CN: 11-4528/TS

Year: 2017

Issue: 2

Volume: 17

Page: 77-84

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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