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The active metabolites of Bacillus subtilis BS08 against bacterium purified from fish were identified by Infrared Spectroscopy and HPLC-MS. Liquid-state fermentation by BS08 was optimized for the maximum antibacterial activity by response surface methodology (RSM). The active metabolites were extracted by acid and alcohol, purified by TLC and identified by Infrared Spectroscopy and HPLC-MS. The analysis revealed the active metabolites were families of lipopeptides, such as surfactin and iturin with molecular mass 995-1 350, thermal and pH stability, widely antimicrobial spectrum. Response surface methodology (RSM) was employed to optimize the cultural conditions of BS08 for the enhancement of lipopeptides. BS08 with 7% inoculum was cultivated at 38 , 200 r/min, pH 8.2 for 24 h in containing yeast extract 14 g/L, glucose 14 g/L, Mg SO41 g/L. The yield of lipopeptides reached 450 mg/L. © 2017, Editorial Office of Journal of CIFST. All right reserved.
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Journal of Chinese Institute of Food Science and Technology
ISSN： 1009-7848
CN： 11-4528/TS
Year： 2017
Issue： 12
Volume： 17
Page： 105-115
Cited Count：
WoS CC Cited Count： 0
SCOPUS Cited Count： 2
ESI Highly Cited Papers on the List： 0 Unfold All
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Chinese Cited Count：
30 Days PV： 2
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