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[期刊论文]

Photoelectrochemical biosensing of disease marker on p-type Cu-doped Zn0.3Cd0.7S based on RCA and exonuclease III amplification

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author:

Zhang, Kangyao (Zhang, Kangyao.) [1] | Lv, Shuzhen (Lv, Shuzhen.) [2] | Lu, Minghua (Lu, Minghua.) [3] | Unfold

Indexed by:

EI

Abstract:

In this work, a new 'signal-on' split-type photoelectrochemical (PEC) sensing platform for prostate-specific antigen (PSA) detection was successfully constructed using p-type Cu-doped Zn0.3Cd0.7S as the photosensitive semiconductor material and target-triggered rolling circle amplification (RCA) for signal amplification. The signal derived from Cu-doped Zn0.3Cd0.7S was amplified by hemin/G-quadruplex. Upon target PSA introduction, the aptamer-primer probe (apt-pri) was captured by capture antibody-conjugated magnetic bead (MB-mAb) to form the sandwiched MB-mAb/PSA/apt-pri. The complex could initiate the RCA reaction to produce a long single-stranded DNA that provided binding sites for G-rich DNA and to form long single-stranded DNA/G-quadruplex/hemin. Upon the addition of exonuclease III (Exo III), the hemin/G-quadruplex immobilized on the RCA long product could be released by the digestion of Exo III. The hemin/G-quadruplex complexes in this study were used as efficient electron acceptors to neutralize the photoelectrons generated from the semiconductor and hindered the recombination of charges, thus enhancing the photocurrent. Under the optimum conditions, the developed sensing system displayed a good analytical performance with a linear range of 0.05–40 ng mL−1 PSA and a detection limit of 16.3 pg mL−1. Furthermore, good selectivity, high anti-interference ability, satisfactory reproducibility, and good accuracy were also achieved. These prominent analytical properties revealed that our strategy might be a potential and reliable tool for the detection of PSA. © 2018 Elsevier B.V.

Keyword:

Amplification Binding sites DNA Photoelectrochemical cells Semiconductor alloys

Community:

  • [ 1 ] [Zhang, Kangyao]Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou; 350116, China
  • [ 2 ] [Lv, Shuzhen]Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou; 350116, China
  • [ 3 ] [Lu, Minghua]Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng; Henan; 475004, China
  • [ 4 ] [Tang, Dianping]Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou; 350116, China

Reprint 's Address:

  • [lu, minghua]institute of environmental and analytical science, school of chemistry and chemical engineering, henan university, kaifeng; henan; 475004, china

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Source :

Biosensors and Bioelectronics

ISSN: 0956-5663

Year: 2018

Volume: 117

Page: 590-596

9 . 5 1 8

JCR@2018

1 0 . 7 0 0

JCR@2023

ESI HC Threshold:209

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

30 Days PV: 0

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