A　highly　sensitive　and　rapid　method　of　in-situ　surface-enhanced　Raman　spectroscopy　(SERS)　combining　with　electrochemical　preconcentration　(EP)　in　detecting　malachite　green　(MG)　in　aquaculture　water　was　established.　Ag　nanoparticles　(AgNPs)　were　synthesized　and　spread　onto　the　surface　of　gold　electrodes　after　centrifuging　to　produce　SERS-active　substrates.　After　optimizing　the　pH　values,　preconcentration　potentials　and　times,　in-situ　EP-SERS　detection　was　carried　out.　A　sensitive　and　rapid　analysis　of　the　low-concentration　MG　was　accomplished　within　200　s　and　the　limit　of　detection　was　2.4　×　10−16　M.　©　2017　Elsevier　B.V.