Pressure-based　bioassays　incorporating　biomolecular　recognition　with　a　catalyzed　gas-generation　reaction　have　been　developed　for　gas　biosensors,　but　most　involve　poor　sensitivity　and　are　unsuitable　for　routine　use.　Herein　we　design　an　innovative　gas　pressure-based　biosensing　platform　for　the　detection　of　Kanamycin　(Kana)　on　polyaniline　nanowires-functionalized　reduced　graphene　oxide　(PANI/rGO)　framework　by　using　platinum　nanozyme-catalyzed　gas　generation.　The　signal　was　amplified　by　coupling　with　catalytic　hairpin　assembly　(CHA)　and　strand-displacement　amplification　(SDA).　Upon　target　Kana　introduction,　the　analyte　initially　triggered　a　SDA　reaction　between　hairpin　DNA1　and　hairpin　DNA2,　and　then　induced　CHA　conjugation　between　magnetic　bead-labeled　hairpin　DNA3　(MB-H3)　and　platinum　nanoparticle-labeled　hairpin　DNA4　(Pt-H4)　to　form　a　three-dimensional　network.　Numerous　platinum　nanoparticles　(peroxidase-like　nanozymes)　were　carried　over　with　magnetic　beads　to　reduce　hydrogen　peroxide　into　oxygen.　The　as-produced　gas　compressed　PANI/rGO　frameworks　(modified　to　polyurethane　sponge,　used　as　the　piezoelectric　materials)　in　a　homemade　pressure-tight　device,　thus　causing　the　increasing　current　of　PANI/rGO　sponge　thanks　to　its　deformation.　The　change　in　the　current　caused　by　the　as-generated　gas　pressure　was　determined　on　an　electrochemical　workstation.　Under　optimum　conditions,　PANI/rGO　sponge　exhibited　outstanding　compressibility,　stable　signal-waveform　output,　fast　response　and　recovery　time　(-109　ms),　and　the　current　increased　with　the　increasing　Kana　concentration　within　a　dynamic　working　range　of　0.2-50　pM　at　a　detection　limit　of　0.063　pM.　Good　reproducibility,　specificity,　and　acceptable　precision　were　acquired　for　Kana　analysis.　In　addition,　the　accuracy　of　this　method　was　monitored　to　evaluate　real　milk　samples　with　the　well-matched　results　obtained　by　using　the　referenced　Kana　ELISA　kit.　Copyright　©　2018　American　Chemical　Society.