Objective:　Analysis　the　relative　properties　of　antifreeze　peptides　from　pigskin　gelatin　and　studying　the　hypothermia　protection　activity　on　the　lactic　acid　bacteria.　Method:　The　ANS　fluorescent　probe　method　was　employed　to　determine　the　surface　hydrophobicity　of　antifreeze　peptides;　The　differential　scanning　calorimetry(DSC)　was　employed　to　determine　the　thermal　hysteresis　activity;　Application　of　polarized　light　microscope　was　used　to　observe　the　recrystallization　inhibition　activity　of　antifreeze　polypeptide;　Detecting　the　survival　rates　of　lactic　acid　bacteria　via　the　plate　colony　count　method　after　frozen.　Results:　the　DH　of　antifreeze　polypeptide　was　7.2%　under　the　optimal　conditions,　and　80%　of　the　components　of　antifreeze　polypeptide　with　the　molecular　mass　distribution　among　150-2　000　u.　The　surface　hydrophobicity　value　of　antifreeze　polypeptide　was　363,　the　isoelectric　point　was　3.93,　and　the　thermal　hysteresis　activity　was　0.58,　respectively.　In　addition,　the　antifreeze　peptides　showed　a　significant　recrystallization　inhibition　activity.　The　result　of　lactic　acid　bacteria　frozen　at　low　temperature　was　shown　that　antifreeze　peptides　provide　a　well　protection　for　the　lactic　acid　bacteria　at　a　concentration　of　1%.　©　2018,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.