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[期刊论文]

Treatment of malachite green by immobilized laccase in ZrO2 via biomineralization

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author:

Wang, Meiyin (Wang, Meiyin.) [1] | Zhang, Xinying (Zhang, Xinying.) [2] (Scholars:张新颖) | Lin, Linlin (Lin, Linlin.) [3] | Unfold

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EI PKU CSCD

Abstract:

A method of laccase immobilization was developed by ZrO2 synthesized via biomineralization, which was prepared by encapsulating laccase in the zirconia nanoparticles with the lysozyme as the inducer, and the recovery rate of enzyme activity was 59%. Field emission scanning electron microscope (FESEM), energy dispersive spectrometer (EDS), and thermal gravimetric analyzer (TGA) were used to characterize ZrO2 nanoparticles before and after the enzyme immobilization. The results proved that laccase was immobilized in ZrO2 successfully and lysozyme was not only induced the formation of zirconia but also acted as a biological template encapsulated by ZrO2. Enzymatic properties of immobilized laccase by biomimetic synthesis were also explored. The optimum pH and temperature of immobilized laccase were 3 and 70, respectively. Compared with free enzyme, the pH and temperature stability of immobilized laccase were significantly increased. The activity of the immobilized laccase was 95% of the original activity after storing for 30 days at 4, and the activity was still 60% of the initial enzyme activity after recycling 5 times. Moreover, the immobilized laccase was used in the decolorization of malachite green and the experimental result showed that the decolorization rate of the malachite green was more than 95% within 6h. Additionally it proved that the immobilized laccase decolorized malachite green by both of adsorption and degradation effects through the UV-visible spectroscopy analysis. © 2018, Chemical Industry Press. All right reserved.

Keyword:

Biomimetic processes Biomineralization Carbonate minerals Enzyme activity Enzyme immobilization Enzymes Nanoparticles Nanostructured materials Radioactive waste vitrification Scanning electron microscopy Spectrometers Synthesis (chemical) Ultraviolet visible spectroscopy Zirconia

Community:

  • [ 1 ] [Wang, Meiyin]College of Environment and Resources, Fuzhou University, Fuzhou; Fujian; 350108, China
  • [ 2 ] [Zhang, Xinying]State Key Laboratory of Energy and Environment Photocatalysis, Fuzhou University, Fuzhou; Fujian; 350002, China
  • [ 3 ] [Lin, Linlin]College of Environment and Resources, Fuzhou University, Fuzhou; Fujian; 350108, China
  • [ 4 ] [Li, Xiaolong]College of Environment and Resources, Fuzhou University, Fuzhou; Fujian; 350108, China
  • [ 5 ] [Li, Zongren]College of Environment and Resources, Fuzhou University, Fuzhou; Fujian; 350108, China

Reprint 's Address:

  • 张新颖

    [zhang, xinying]state key laboratory of energy and environment photocatalysis, fuzhou university, fuzhou; fujian; 350002, china

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Source :

Chemical Industry and Engineering Progress

ISSN: 1000-6613

CN: 11-1954/TQ

Year: 2018

Issue: 7

Volume: 37

Page: 2798-2805

Cited Count:

WoS CC Cited Count: 0

30 Days PV: 3

Online/Total:119/10284588
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